Journal: bioRxiv
Article Title: Intracellular Delivery of Full-Length Antibodies via Poly-L-lysine-Coated PEG–PLGA Polymersomes Enables Non-invasive Pulmonary Immunotherapy
doi: 10.1101/2025.09.16.676558
Figure Lengend Snippet: (A) IVIS images and IHC-stained lung sections from animals treated with AF488-labeled IgG antibody– loaded PLL-PS or saline. (B) In vivo cytotoxicity analysis of lungs treated with AF488-labeled IgG antibody–loaded PLL-PS or saline, assessed by IHC staining for cleaved Caspase-3 expression. Results represented as Mean ± SEM.
Article Snippet: PLL 30KDa (Sigma-Aldrich, P2636); Anti-human IgG AF488 (SoutherenBiotech; 2030-40); Primary human Lung Fibroblasts (ATCC, PCS-201-013); Fibroblast Basal Media (ATCC, PCS-201-030); Fibroblast growth Kit (ATCC, PCS-201-041), NLRP3 Ab (Novus Biologicals; NBP2-12446); Anti mouse Caspase 3 antibody, Anti-Rabbit IgG Cy5 conjugated, MTT assay kit (Invitrogen, V13154), IL-1β Elisa Kit (Invitrogen, DY201-05), DAPI (Invitrogen, D 3571), BCA assay Kit (Thermo Fischer, 23227), Uranyl Acetate, TEM Grid, 300 kDa Float-A-Lyzer (Biotech CE Trial kit dialysis membrane).
Techniques: Staining, Labeling, Saline, In Vivo, Immunohistochemistry, Expressing